Call Prefix : PR-AS

Call Suffix : 1998

Call Cutter : JARQ V32N1

Location : SEARCA Library

Format : Serials

Series : Japan Agricultural Research Quarterly (JARQ)

Publisher : Japan International Research Center for Agricultural Science (JIRCAS),

Publication Date : January 1998

Publication Place : Tsukuba, Ibaraki, Japan:

Summary : DFA I (difructose dianhydride I) is a dissacharide composed of 2 molecules of fructose. DFA I is generated from inulin by the action of an enzyme produced by a bacterium Arthrobacter globiformis S14-3. The gene encoding the enzyme was cloned and the nucleotides sequenced, for the first time. The sequence indicated that the native enzyme is composed of 392 amino acid residues. The 1.5 Kb DNA fragment encoding the gene was found to produce the active enzyme, under the control of the lac promoter of pUC 119.