Author : Nguyen Thai Van

Summary : The experiments were conducted to determine the possible uses of tissue culture on papaya research specially as a method of vegetative propagation. Three growth regulators namely : Indole butyric Acid (IBA), Gibberellic Acid (GA) and Benzyl Adenine (BA) were shown to enhance the formation and growth of papaya callus. 2,4-Dichlorophenoxy acetic acid (2,4-D) at very low level also promoted the development of papaya callus. Coconut water was found to be helpful on the promotion of the callus formation of papaya tissue. However, the combination of all these substances did not surpass the treatment combination of coconut water, tyrosine and 2,4-D. In one instance, the latter combination stimulated the formation of roots from papaya callus. Inositol potassium phosphate levels and the change on different nutrient components in some media and also the absic regulatory mechanism involving a balance between auxin and cytokinin did not seem applicable for the initiation of roots or shoots on papaya tissue cultured in vitro.