Author : Woro Wulandari Kalanjati

Summary : Chicken slaughterhouses play an important role in the spread of ESBLproducing
E. coli into the meat supply chain for the consumers. The aim of the
study was to detect the occurrence of ESBL-producing E. coli in the environment
of the chicken slaughterhouses in Bogor. ESBL-producing E. coli isolates (n=20)
were collected from 84 samples of the environment. Isolates obtained were
identified using API 20E™ kit. Samples were collected from seven different
locations for a period time of a week. ESBL in this study was confirmed using
MAST® D68C kit. A total of 20 ESBL-producing E. coli isolates were tested .
against 18 antimicrobial agents using the disc diffussion method and the
interpretation of resistance was conducted according to Clinical and Laboratory
Standards Institute (CLSI) guidelines. ESBL-producing E. coli isolates showed
resistance against all of 18 antimicrobial agents and 100% of the isolates showed
resistance against more than 9 antibiotics (multidrug resistance). The isolates were
resistant to penicilin (100%), amoxicillin (100%), ampicillin (100%), cefotaxim
(100%), cefpodozime (100%), ceftazidim (100%), streptomycin (95%),
gentamycin (85%), trimetoprim-sulphamethoxazole (85%), tetracycline (70%),
kanamycin (65%), ciprofloxacine (60%), norfloxacine (55%), doxycycline (45%),
colistin sulfate (45%), cephalothin (30%), neomycin ·(15%), and polymyxin B
(15%).

ESBL-producing E. coli bacteria derived from environment of chicken
slaughterhouses (n=20) were found on the carcasses containers (20%), offal
containers (10%), floor of carcasses and offal handling area (25%), knives (15%),
tank water (5%) and feathers puller machine (25%). The existence of resistant
bacteria in the environment of chicken slaughterhouses could lead to transmission
of resistance genes to other bacteria that could spread to the carcass that was
potentially harmful to humans.