Call Prefix : PR-T

Call Suffix : 2017

Call Cutter : T - Vete 20

Location : SEARCA Library

Format : TD

Publisher : Institut Pertanian Bogor

Publication Date : July 2017

Publication Place : Bogor, Indonesia

Summary : Chicken slaughterhouses play an important role in the spread of ESBLproducing E. coli into the meat supply chain for the consumers. The aim of the study was to detect the occurrence of ESBL-producing E. coli in the environment of the chicken slaughterhouses in Bogor. ESBL-producing E. coli isolates (n=20) were collected from 84 samples of the environment. Isolates obtained were identified using API 20E™ kit. Samples were collected from seven different locations for a period time of a week. ESBL in this study was confirmed using MAST® D68C kit. A total of 20 ESBL-producing E. coli isolates were tested . against 18 antimicrobial agents using the disc diffussion method and the interpretation of resistance was conducted according to Clinical and Laboratory Standards Institute (CLSI) guidelines. ESBL-producing E. coli isolates showed resistance against all of 18 antimicrobial agents and 100% of the isolates showed resistance against more than 9 antibiotics (multidrug resistance). The isolates were resistant to penicilin (100%), amoxicillin (100%), ampicillin (100%), cefotaxim (100%), cefpodozime (100%), ceftazidim (100%), streptomycin (95%), gentamycin (85%), trimetoprim-sulphamethoxazole (85%), tetracycline (70%), kanamycin (65%), ciprofloxacine (60%), norfloxacine (55%), doxycycline (45%), colistin sulfate (45%), cephalothin (30%), neomycin ·(15%), and polymyxin B (15%). ESBL-producing E. coli bacteria derived from environment of chicken slaughterhouses (n=20) were found on the carcasses containers (20%), offal containers (10%), floor of carcasses and offal handling area (25%), knives (15%), tank water (5%) and feathers puller machine (25%). The existence of resistant bacteria in the environment of chicken slaughterhouses could lead to transmission of resistance genes to other bacteria that could spread to the carcass that was potentially harmful to humans.